PRELIMINARY STUDY OF GUT BACTERIAL ABUNDANCE IN Rhynchophorus ferrugineus (COLEOPTERA: DRYOPHTHORIDAE) FED ON DIFFERENT DIETS

Farah Nadiah Rosli, Norefrina Shafinaz Md Nor, Nurul Wahida Othman

Abstract


Rhynchophorus ferrugineus or also known as RPW is a major pest in many parts of the world and has infested coconut plantations in Malaysia especially in Terengganu and Kelantan. To date, a combination of many methods such as trapping with pheromone, chemical pesticide, and biological pesticide (Integrated Pest Management) were applied to control RPW infestation. However, IPM often could not be used as a method for total eradication and in addition to that, IPM necessitates continuous laborious effort. Thus, a new target for RPW eradication method with high specificity must be identified. The purpose of this research is to study the effect of different diets towards gut bacterial abundance in RPW and thus widen the knowledge regarding their correlation. Parameter measured were abundance of gut bacteria following different diets treatment. 16S rRNA amplicon sequencing method was used in this research to identify all gut bacteria present in the RPW. Our analysis showed that there are six most abundant group of bacteria with different phylogenetic group rank present in RPW’s gut. This include Enterobacteriaceae, Leminorella grimontii, Entomoplasmatales, Erysipelothrix, Lactobacillus and Leuconostoc. This groups of bacteria were known to have significant roles toward host’s gut such as aiding in digestion, synthesizing hormone, and protecting from pathogenic bacteria growth. Comprehensive data obtained from this study on these microbes have potential to be used in exploring new dimension of RPW pest management.


Full Text:

PDF

References


Azmi, W.A., Chik, Z. & Abdul Rahman, A. 2013. A new invasive coconut pest in Malaysia: the red palm weevil (Curculionidae: Rhynchophorus ferrugineus). Planter 89(1043): 97-110.

Brauman, A., Doré, J., Eggleton, P., Bignell, D., Breznak, J.A. & Kane, M.D. 2001. Molecular phylogenetic profiling of prokaryotic communities in guts of termites with different feeding habits. FEMS Microbiology Ecology 35(1): 27-36.

Cai, H., Rodriguez, B. T., Zhang, W., Broadbent, J. R. & Steele, J.L. 2007. Genotypic and phenotypic characterization of Lactobacillus casei strains isolated from different ecological niches suggests frequent recombination and niche specificity. Microbiology 153(8): 2655-2665.

Chapman, R.F. 1998. The Insects: Structure and Function. Cambridge: Cambridge University Press.

Cochran, D.G. 1985. Nitrogen excretion in cockroaches. Annual Review of Entomology 30(1): 29-49.

Colman, D.R., Toolson, E. C. & Takacs‐Vesbach, C. 2012. Do diet and taxonomy influence insect gut bacterial communities? Molecular Ecology 21(20): 5124-5137.

De Vries, J., Jacobs, G., Sabelis, M.W., Menken, S.B. & Breeuwer, J.A. 2004. Diet dependent effects of gut bacteria on their insect host: the symbiosis of Erwinia sp. and western flower thrips. Proceedings of the Royal Society of (B: Biological Sciences, London) 2171- 2178.

Dillon, R., Vennard, C., Buckling, A. & Charnley, A. 2005. Diversity of locust gut bacteria protects against pathogen invasion. Ecology Letters 8(12): 1291-1298.

Douglas, A.E. 2009. The microbial dimension in insect nutritional ecology. Functional Ecology 23(1): 38-47.

El-Juhany, L.I. 2010. Degradation of date palm trees and date production in Arab countries: causes and potential rehabilitation. Australian Journal of Basic and Applied Sciences 4(8): 3998-4010.

El-Shafie, H., Faleiro, J., Al-Abbad, A., Stoltman, L., & Mafra-Neto, A. 2011. Bait-free attract and kill technology (Hook™ RPW) to suppress red palm weevil, Rhynchophorus ferrugineus (Coleoptera: Curculionidae) in date palm. Florida Entomologist 94(4): 774-778.

Funaro, C.F., Kronauer, D.J., Moreau, C.S., Goldman- Huertas, B., Pierce, N.E. & Russell, J. A. 2011. Army ants harbor a host-specific clade of Entomoplasmatales bacteria. Applied and Environmental Microbiology 77(1): 346-350.

French, A.S., Simcock, K.L., Rolke, D., Gartside, S.E., Blenau, W. & Wright, G.A. 2014. The role of serotonin in feeding and gut contractions in the honeybee. Journal of Insect Physiology61: 8-15

Gush, H. 1997. Date with disaster. The Gulf Today September: 16.

Harris, M.N., Norzainih, J. & Wahida, O.N. 2015. Morphology and histology of the digestive System of the red palm weevil larva, Rhynchophorus Ferrugineus, Olivier (Coleoptera: Dryophthoridae). Proceedings of the 3rd International Conference on Chemical, Agricultural and Medical Sciences (Singapore).

Harris, M.N, Norefrina Shafinaz, M.N., Salmah, Y. & Nurul Wahida, O. 2016. Distribution of serotonin (5-HT) and dopamine (DA) on digestive tract of red palm weevil larva, Rhynchophorus ferrugineus (Coleoptera: Dryophthoridae). Serangga 21(1): 39-50.

Ince, S., Porcelli, F. & Al-Jboory, I. 2011. Egg laying and egg laying behavior of red palm weevil Rhynchophorus ferrugineus (Olivier) 1790 (Coleoptera: Curculionidae), Agriculture and Biology Journal of North 2: 1368-1374.

Murphy, S. & Briscoe, B. 1999. The red palm weevil as an alien invasive: biology and the prospects for biological control as a component of IPM. Biocontrol News and Information 20: 35N-46N.

Musgrave, A. 1964. Insect mycetomes. The Canadian Entomologist 96(1-2): 377-389.

Nicholson, J.K., Holmes, E., Kinross, J., Burcelin, R., Gibson, G., Jia, W. & Pettersson, S. 2012. Host-gut microbiota metabolic interactions, Science 336(6086): 1262-1267.

Potrikus, C.J. & Breznak, J.A. 1981. Gut bacteria recycle uric acid nitrogen in termites: a strategy for nutrient conservation. Proceedings of the National Academy of Sciences 78(7): 4601-4605.

Sandine, W.E. 1979. Roles of Lactobacillus in the intestinal tract. Journal of Food Protection 42(3): 259-262.

Ulrich, R., Buthala, D. & Klug, M. 1981. Microbiota associated with the gastrointestinal tract of the common house cricket, Acheta domestica. Applied and Environmental Microbiology 41(1): 246-254.

Vega, F.E. & Dowd, P.F. 2005. The role of yeasts as insect endosymbionts. In Vega, F.E. & Blackwell (eds.). Insect-Fungal Associations: Ecology and Evolution, pp. 211- 243. New York: Oxford University Press.


Refbacks

  • There are currently no refbacks.