THE INTEGRATED VECTOR MANAGEMENT OF THE SYNERGISM FORMULATION BETWEEN Ipomeoa cairica PLANT EXTRACT AND Metarhizium anisopliae META-G4 ON Aedes MOSQUITOES
Abstract
The integrated vector management (IVM) is a new concept to optimize the use of resources to maximize the efficacy and sustainability of the vector control. Considering the excellent performances of Metarhizium anisopliae fungi and Ipomoea cairica leaf extract against Aedes larvae, here we investigated the possibility of integrated synergism effects of these two natural agents into one formulation. The larvicidal effectiveness and the persistence of this formulation were tested against primary and secondary vectors of dengue fever, Aedes aegypti and Ae. albopictus. The study revealed the compatibility of I. cairica leaf extracts and M. anisopliae fungi was at the maximum level of 450 ppm and 6 × 106 conidia/mL, respectively, in which were able to endure the spore production of M. anisopliae by estimating using curves estimation regression models. The synergistic effects of these two integrated agents had shown high susceptibility and caused faster larval mortality for Aedes albopictus at 100% within 6 days of the treatment periods and 15 days for Ae. aegypti. The effectiveness of these integrated agents was reduced to less than 50% after day 21. The current finding shows that the combination of M. anisopliae with I. cairica can produce strong synergistic interaction justified the possibilities of combining these two agents into one formulation and can prolong the effectiveness in controlling the dengue vectors. In facts, the effectiveness of this formulation towards both Aedes larvae has approved the potential to be used in IVM to curb dengue diseases.
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