PERKEMBANGAN DAN CIRI-CIRI UTAMA INTRAPUPARIA Spiniphora genitalis SCHMITZ, 1940 (DIPTERA: PHORIDAE) PADA SUHU BILIK

Hemavashini Krebanatham, Reena Abd. Rashid, Raja Muhammad Zuha

Abstract


Bidang entomologi forensik lazimnya menggunakan perkembangan spesimen Diptera yang paling lama berada pada mayat untuk menganggar selang masa pasca kematian minimum (Postmortem Intervalmin – PMImin). Walaupun pupa adalah peringkat metamorfosis yang paling lama berlaku dalam kitar hidup Diptera, maklumat mengenai perkembangan pupa sebagai kronometri biologi untuk digunakan dalam siasatan forensik amatlah terhad. Kekurangan ini dapat diatasi dengan memperihalkan perkembangan intrapuparia berdasarkan peristiwa yang berlaku sepanjang proses metamorfosis dalam puparium. Objektif kajian ini adalah untuk memperihalkan perkembangan pupa Spiniphora genitalis Schmitz, 1940 (Diptera: Phoridae) pada suhu bilik berdasarkan perkembangan intrapuparia. Larva S. genitalis yang diperoleh daripada koloni peliharaan Makmal Entomologi Forensik, Fakulti Sains Kesihatan, Universiti Kebangsaan Malaysia (UKM), Malaysia, dipelihara pada hati lembu di suhu bilik 25°C – 37°C. Pada permulaan peringkat pempupaan, pupa disampel pada selang masa 3, 6 dan 12 jam, dan kemudiannya puparium dipisahkan dengan teliti dan dianalisis secara stereomikroskopi. Dalam kajian ini, sejumlah 14 peringkat perkembangan dikenal pasti bagi pupa S. genitalis, dengan jumlah tempoh perkembangan intrapuparia adalah 240 – 252 jam sebelum menjadi lalat dewasa. Ciri-ciri utama morfologi intrapuparia turut dihasilkan yang mana ia menunjukkan warna mata majmuk dan gabungan beberapa ciri-ciri lain, dapat dijadikan penunjuk berguna untuk menentukan usia pupa bagi anggaran PMImin dalam entomologi forensik.


Full Text:

PDF

References


Barros-Cordeiro, K.B., Pujol-Luz, J.R., Name, K.P.O. & Báo, S.N. 2016. Intra-puparial development of the Cochliomyia macellaria and Lucilia cuprina (Diptera, Calliphoridae). Revista Brasileira de Entomologia 60: 334–340.

Beaver, R. 1987. Biological studies of muscoid flies (Diptera) breeding in mollusc carrion in Southeast Asia. Medical Entomology and Zoology 37: 205–211.

Brown, K., Thorne, A. & Harvey, M. 2015. Calliphora vicina (Diptera: Calliphoridae) pupae: A timeline of external morphological development and a new age and PMI estimation tool. International Journal of Legal Medicine 129(4): 835–850.

Da Silva, S.M. & Moura, M.O. 2019. Intrapuparial development of Hemilucilia semidiaphana (Diptera: Calliphoridae) and its use in forensic entomology. Journal of Medical Entomology 56(6): 1623–1635.

Disney R H L. 1994. Scuttle Flies: The Phoridae. London: Chapman & Hall.

Disney, R.H.L. & Bänziger, H. 2009. Further records of scuttle flies (Diptera : Phoridae) imprisoned by Aristolochia baenzigeri (Aristolochiaceae) in Thailand. Journal of the Swiss Entomological Society (82): 233–251.

Feng, D.-X. & Liu, G.-C. 2013. Pupal age estimation of forensically important Megaselia spiracularis Schmitz (Diptera: Phoridae). Forensic Science International 231(1–3): 199–203.

Flissak, J.C. & Moura, M.O. 2018. Intrapuparial development of Sarconesia chlorogaster (Diptera: Calliphoridae) for postmortem interval estimation (PMI). Journal of Medical Entomology 55(2): 277–284.

Hall, M.J.R. & Martin-Vega, D. 2019. Visualization of insect metamorphosis. Phisolophical Transactions of the Royal Society B 374: 20190071.

Joyce, C.R. 1967. Proceedings of the Hawaiian Entomological Society for 1966. Hawaiian Entomological Society. Honolulu, Hawaii 19(3): 334.

Karabey, T. & Sert, O. 2014. The analysis of pupal development period in Lucilia sericata (Diptera: Calliphoridae) forensically important insect. International Journal of Legal Medicine 132: 1185–1196.

Lee, Y.M., Disney, R.H.L. & Zuha, R.M. 2021. Development of forensically important scuttle fly, Megaselia spiracularis Schmitz (Diptera: Phoridae) at ambient temperatures with distinguishing features of the larval instars. Journal of Asia-Pacific Entomology 24(3): 858–865.

Li, L., Wang, Y. & Wang, J. 2016. Intra-puparial development and age estimation of forensically important Hermetia illucens (L.). Journal of Asia-Pacific Entomology 19(1): 233–237.

Oliva, A. 2004. Insects of forensic interest in Buenos Aires (Argentina). Addenda et corrigenda. I. Phoridae (Diptera: Brachycera), Physis (Buenos Aires), Sección C. 60(138–139): 45–50.

Schmitz, H., 1940. Eine neue ostasiatische Spiniphora (Phoridae, Diptera). Natuurhistorisch Maandblad 29(8): 78-79.

Sharanya, B. & Zuha, R.M. 2019. A preliminary geometric morphometric assessment of two forensically important blow fly larvae in Malaysia, Chrysomya megacephala (Fabricius) and Chrysomya rufifacies (Macquart) (Diptera: Calliphoridae). Serangga 24(1):70–79.

Thyssen, P.J., Aquino, M.F., Purgato, N.C., Martins, E., Costa, A.A., Lima, C.G. & Dias, C.R. 2018. Implications of entomological evidence during the investigation of five cases of violent death in Southern Brazil. Journal of Forensic Science and Research 2(1): 001–008.

Wells, J.D. & LaMotte, L. R. 2020. Chapter 9: Estimating the postmortem interval. Dlm. Byrd, J.H. & Tomberlin, J.K. (pnyt.) Forensic Entomology – the Utility of Arthropods in Legal Investigations, hlm. 213 – 224. Boca Raton: CRC Press.

Zhang, Y., Wang, Y., Yang, L., Tao, L. & Wang, J. 2018. Development of Chrysomya megacephala at constant temperatures within its colony range in Yangtze River Delta region of China. Forensic Sciences Research 3(1): 74–82.

Zuha, R.M. & Omar, B. 2014. Developmental rate, size, and sexual dimorphism of Megaselia scalaris (Loew) (Diptera: Phoridae): Its possible implications in forensic entomology. Parasitology Research 113(6): 2285-2294.

Zuha, R.M., Ankasha, S.J., Disney, R.H.L. & Omar, B. 2015. Indoor decomposition study in Malaysia with special reference to the scuttle flies (Diptera: Phoridae). Egyptian Journal of Forensic Sciences 6(3): 216–222.

Zuha, R.M., See, H.-W., Disney, R.H.L. & Omar, B. 2017. Scuttle flies (Diptera: Phoridae) inhabiting rabbit carcasses confined to plastic waste bins in Malaysia include new records and an undescribed species. Tropical Life Sciences Research 28(1): 131–143.


Refbacks

  • There are currently no refbacks.