EFFECT OF LIQUID FORMULATION OF Metarhizium anisopliae (COMPOST, METANKOS) ON MORTALITY OF Oryctes rhinoceros L. LARVAE ON OIL PALM

Hafiz Fauzana, Nelvia Nelvia, Rusli Rustam, Fifi Puspita, Juliana Azzahra, Mukhlis Ibrahim

Abstract


Palm oil trees are widely cultivated in Riau Province producting large quantities of oil palm empty fruit bunches (EFB) that can be composted and enriched with the biological control agent Metarhizium anisopliae Metsch. to manage the pest Oryctes rhinoceros L. This study aims to obtain the best dosage of the liquid formulation of M. anisopliae in Metankos on the mortality of O. rhinoceros larvae in oil palm plants. This research was conducted experimentally using a complete randomized design (CRD). The data obtained were analyzed statistically using the SAS software, followed by DNMRT test at 5% significance level. The results showed that the best dose of M. anisopliae liquid formulation on compost media was 210 g/l per bucket with a total mortality of 90%. A dose of 120 g/L per the bucket also produced significant mortality (72.5%), meeting the criteria of a bioinsecticide for controlling O. rhinoceros larvae. These findings indicate that the Metankos liquid formulation has strong potential as an effective biological control agent in oil palm pest management.


Full Text:

PDF

References


Abdullah, T., Aminah, S.N. & Annisaa, N.W. 2021. The mortality of green plant hoppers Nephotettix virescens (Homoptera: Cicadellidae) by Metarhizium anisopliae Metsch and Beauveria bassiana (Bals.) Vuill. IOP Conference Series: Earth and Environmental Science 807(2): 022-107.

Archana, H.R., Darshan, K., Lakshmi, M.A., Ghoshal, T., Bashayal, B.M. & Aggarwal, R. 2022. Biopesticides: A key player in ago-environmental sustainability. In. Ravindra S., Deep, C.S., Ajar, N.Y. & Reeta, G. (eds.). Trends of Applied Microbiology for Sustainable Economy, pp. 613–653. India : Elsevier.

Baleba, S.B., Agbessenou, A., Getahun, M.N., Subramanian, S. & Masiga, D. 2021. Infection of the Stable Fly, Stomoxys calcitrans by the entomopathogenic fungi Metarhizium anisopliae (Hypocreales: Clavicipitaceae) negatively affects its survival, feeding propensity, fecundity, fertility, and fitness parameters. Frontiers in Fungal Biology 2(6): 37-81.

Central Bureau of Statistics. 2023. Plantation Crop Area by Province. Central Statistics Agency Riau.

https://www.bps.go.id/id/statistics-table/2/MTMxIzI=/plantation-area-by-province.html [22 October 2024]

Eilenberg, J., Saussure, S., Ben Fekih, I., Jensen, A.B. & Klingen, I. 2019. Factors driving susceptibility and resistance in aphids that share specialist fungal pathogens. Current Opinion in Insect Science 33: 91–98.

Erawati, D.N. & Wardati, I. 2016. Biocontrol technology of Metarhizium anisopliae and Beauveria bassiana against palm oil weevil (Oryctes rhinoceros). National Seminar on Research Results and Community Service, pp 1–5.

Fauzana, H., Arda, F., Nelvia, Rustam, R. & Puspita, F. 2020. Test on several Metarhizium anisopliae (Metsch) Sorokin on centrations in palm oil empty fruit bunch compost (Metankos) to infect Oryctes rhinoceros larvae. Journal of Physics: Conference Series 1655 (1): 12021.

Fauzana, H. & Fadilla, M. 2022. Test of Metarhizium anisopliae (Metsch.) concentration increase in compost media in controlling Oryctes rhinoceros L. larvae. Journal of Agotechnology 12(2): 65.

Fauzana, H., Nelvia, Rustam, R., Puspita, F. & Feronica, R. 2023. The dosage of biopesticide formulation of local Metarhizium anisopliae flour in compost (Metankos) against Oryctes rhinoceros L. IOP Conference Series: Earth and Environmental Science 1160(1): 012046.

Handoko, J., Fauzana, H. & Sutikno, A. 2017. Population and intensity of horn beetle (Oryctes rhinoceros Linn.) attack on immature palm oil (Elaeis guineensis Jacq.) plants. JOM Faperta Universitas Riau 4(1): 155–169.

Hasnah & Susana, H.S. 2012. The effectiveness of the Beauveria bassiana Vuill fungus against the mortality of the green ladybug Nezara viridula L. on stadia nimfa and imago. Floratek 7(1): 13–24.

Hasyim, A. 2013. Increased infectivity of entomopathogenic fungus, Beauveria bassiana (Balsamo) Vuill. on various carrier materials to control banana weevil borer, Cosmopolites sordidus Germar in the field. Journal of Horticulture 17(4): 85653.

Herlinda, S., Hartono & Irsan, C. 2008. Efficacy of bioinsecticide liquid formulation with active ingredients Beauveria bassiana (Bals.) Vuill. and Metarhizium sp. PATPI National Seminar and Congress 2008, Palembang 14-16 October 2008, pp. 1–15.

Herlinda, S., Sari, E.M., Pujiastuti, Y., Suwandi, Nurnawati, E. & Riyanta, A. 2005. Variation of virulence of Beauveria bassiana (Bals.) Vuill. strains against Plutella xylostella (L.) larvae (Lepidoptera : Plutellidae). Agritrop 24(2): 52–57.

Indriyanti, D.R., Anggaeni, S.D. & Slamet, M. 2017a. Density and composition of Oryctes rhinoceros (Coleoptera: Scarabaeidae) stadia in the field. ARPN Journal of Engineering and Applied Sciences 12(22): 6364–6371.

Indriyanti, D.R., Damayanti, Beautiful, B., Setiati, N. & Priyono, B. 2017b. Mortality and tissue damage for each symptom of Oryctes rhinoceros L. larvae infection due to treatment of the Metarhizium anisopliae fungi. Life Sciences 6(1): 9–17.

Islam, W., Adnan, M., Shabbir, A., Naveed, H., Abubakar, Y.S., Qasim, M., Tayyab, M., Noman, A., Nisar, M.S., Khan, K.A. & Ali, H. 2021. Insect-fungal-interactions: A detailed review on entomopathogenic fungi pathogenicity to combat insect pests. Microbial Pathogenesis 159: 105122.

Jiang, W., Peng, Y., Ye, J., Wen, Y., Liu, G. & Xie, J. 2020. Effects of the entomopathogenic fungus Metarhizium anisopliae on the mortality and immune response of Locusta migatoria. Insects 11(1): 36.

Lestari, R.J., Okalia, D. & Ezward, C. 2020. Analysis of P, K, Ca, and Mg content in composting Tritankos (tricho empty fruit bunches) enriched with cow manure. Geen Swarnadwipournal 9(1): 93–101.

Lin, G., Tanguay, A., Guertin, C., Todorova, S. & Brodeur, J. 2017. A new method of loading predatory mites with entomopathogenic fungi for biological control of their prey. Biological Control 115: 105–111.

Litwin, A., Nowak, M. & Różalska, S. 2020. Entomopathogenic fungi: Unconventional applications. Reviews in Environmental Science and Biotechnology 19(1): 23–42.

Nasution, L., Corah, R., Nuraida, N. & Siregar, A.Z. 2018. Effectiveness of Trichoderma and Beauveria bassiana on larvae of Oryctes rhinoceros on palm oil plant (Elaeis guineensis Jacq.) in vitro. International Journal of Environment, Agriculture and Biotechnology 3(1): 158–169.

Nuryanti, N.S.P., Wibowo, L. & Azis, A. 2012. Addition of several types of nutrient materials to the propagation media to increase the virulence of Beauveria bassiana against walang sangit pests. Journal of Pests and Diseases of Tropical Plants 12(1): 64–70.

Prayogo, Y., Tengkano, W. & Marwoto. 2005. Prospek cendawan entomopatogen Metarhizium anisopliae untuk mengendalikan ulat grayak Spodoptera litura pada kedelai. Jurnal Libang Pertanian 24(1): 19-26.

Purnamayani, R. 2013. Technology For Making Palm Oil Empty Bunch Compost. Jambi: Agricultural Technology Assessment Center.

Rahayuwati, S. & Kusuma, Y.M. 2024. Comparative study of sawdust media and fermented mixture of sawdust-cow dung for rearing Oryctes rhinoceros (Coleoptera: Scarabaeidae) larvae. Serangga 29(4): 142-155.

Salbiah, D., Hennie Laoh, J. & Nurmayani. 2013. Test of several doses of Beauveria bassiana Vuillemin against Oryctes rhinoceros (Coleoptera; Scarabaeidae) horn beetle larvae in palm oil. Journal of Technobiology IV 4(2): 137–142.

Santi, I.S., Ahmad, N.F., Elfatma, O. & Hasanah, N.A.U. 2022. Bio-use power of insecticide Metarizhium anisopliae in controlling Oryctes rhinoceros in palm oil. Tropical Plantation Journal 1(1): 30–34.

Sihombing, R., Oemry, S. & Lubis, L. 2014. Testing the effectiveness of several entomopathogens on Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) larvae in the laboratory. Journal of Agroecotechnology, University of North Sumatra 2(4): 100698.

Simamora, L.O., Bakti, D., Oemry, S. & Manik, F. 2013. Epizootic study of Metarhizhium anisopliae on barnacle Larvae (Plutella xylostella L.) (Lepidoptera: Plutellidae) in greenhouses. Journal of Agroecotechnology Online 1(2): 166–177.

Siswanto & Trisawa, I.M. 2017. Quality and Effectiveness Test of Metarhizium anisopliae Isolated From Central Kalimantan Against Oryctes rhinoceros (Coleoptera: Scarabaeidae). Bogor: Research Report, Plantation Research and Development Center.

Sterkel, M., Haines, L.R., Casas-Sánchez, A., Adung'a, V.O., Vionette-Amaral, R.J., Quek, S., Rose, C., dos Santos, M.S., Escude, N.G., Ismail, H.M., Paine, M.I., Barribeau, S.M., Wagstaff, S., MacRae, J.I., Masiga, D., Yakob, L., Oliveira, P.L. & Acosta-Serrano, Á. 2021. Repurposing the orphan drug nitisinone to control the transmission of African trypanosomiasis. PLoS Biology 19(1): e3000796.

Sun, Y., Hong, S., Chen, H., Yin, Y. & Wang, C. 2022. Production of helvolic acid in Metarhizium contributes to fungal infection of insects bacteriostatic inhibition of the host cuticular microbiomes. Microbiology spectrum 10(5): e02620-22.

Suprayogi, Marheni & Oemry, S. 2015. Effectiveness test of entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae against green ladybugs. Journal of Agoecotechnology Online 3(1): 320–327.

Suryanto, T. 2020. Effectiveness test of Metarhizium anisopliae as control of Oryctes rhinoceros larvae in palm oil plantations. Journal of Citra Widya Education 12(2): 143-148.

Syazwan, S.A., Lee, S.Y., Sajap, A.S., Lau, W.H., Omar, D. & Mohamed, R. 2021. Interaction between Metarhizium anisopliae and its host, the subterranean termite Coptotermes curvignathus during the infection process. Biology 10(4): 263.

Triasih, U., Agustina, D.D.M.E. & Wuryantini, S. 2019. Testing various carriers for viability and conidia density in several liquid biopesticides of entomopathogenic fungi testing various carriers for viability and conidia density in several liquid biopesticides of entomopathogenic fungi. Jurnal Agronida 5(1): 12–20.

Umar, W.K., Agustiyar, F. & Rahma, A. 2022. Verification of the Walkley Black method test for determination of organic carbon elements in palm oil empty fruit bunch waste fertilizer. Exergy 19(3): 97–103.

Wahyudi, P. 2002. Pathogenicity test of the entomopathogenic fungus Beauveria bassiana Vuill. against caterpillars (Spodoptera litura). Biosphere 19: 1–5.

Wahyuni, L., Darma, S. & Wayahdi, M.R. 2017. An expert system identifies symptoms of nutrient deficiency in palm oil plants. National Seminar on Informatics (SNIf) 1(1): 216–222.

Widians, J.A. & Rizkyani, F.N. 2020. Identification of oil palm pests using the certainty factor method. ILKOM J. Ilmiah 12(1): 58-63.


Refbacks

  • There are currently no refbacks.